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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: WSS25 Inhibits Growth of Xenografted Hepatocellular Cancer Cells in Nude Mice by Disrupting Angiogenesis via Blocking Bone Morphogenetic Protein (BMP)/Smad/Id1 Signaling
doi: 10.1074/jbc.M110.105544
Figure Lengend Snippet: WSS25 potently inhibited the expression of Id1. A, HMEC-1 cells were treated with WSS25 (25 μg/ml) for 18 h. RNA was then extracted for the oligo-angiogenesis microarray analysis. Circles indicate Id1. B, WSS25 at 25 μg/ml nearly completely inhibited the Id1 mRNA expression in HMEC-1 cells with 18 S rRNA as the internal control. C, WSS25 down-regulated the Id1 protein expression in HMEC-1 cells in a dose-dependent manner. β-Actin was used as a control for protein loading. Except for the microarray analysis, all experiments were repeated three times.
Article Snippet: The purified cRNA probes were then hybridized to the pretreated
Techniques: Expressing, Microarray